- ACLT stands for Anterior Cruciate Ligament Transection (surgical procedure)
- ACLT stands for African Caribbean Leukaemia Trust (blood and bone marrow donation; UK)
- ACLT stands for Anchorage Community Land Trust (est. 2003; Anchorage, AK)
- ACLT stands for American Chestnut Land Trust
- ACLT stands for Accelerated Cable Life Test
- ACLT stands for Aéro-Club de la Tranche-sur-Mer (French air club)
- ACLT stands for Advanced Community Law Training
- ACLT stands for Actual Calculated Landing Time
- ACLT stands for Albany Community Land Trust (Albany, NY)
- ACLT stands for Association Canadienne de Loisir Therapeutique (French: Canadian Therapeutic Recreation Association)
- ACLT stands for Ashland Community Land Trust (est. 1998; Ashland, OR)
- ACLT stands for Associated Contract Loggers and Truckers (of Minnesota)
- ACLT stands for Acquisition Lead Time
ACLT