- ACNO stands for Arts Council of New Orleans (Louisiana)
- ACNO stands for Association des Comites Nationaux Olympiques (French: Association of the National Olympic Committees)
- ACNO stands for Assistant Chief of Naval Operations
- ACNO stands for Assistant Chief Nursing Officer
- ACNO stands for Accession Number
- ACNO stands for Anti-Cancer Number One (Chinese herb formula)
ACNO