- ACOT stands for A Cherry on Top
- ACOT stands for Apple Classrooms of Tomorrow
- ACOT stands for Average Cups of Tea (song)
- ACOT stands for American College of Technology (Saint Joseph, MO)
- ACOT stands for Atlantic Coast Old Timers
- ACoT stands for Associateship of the College of Teachers (UK)
- ACOT stands for Advanced Communications-Computer Officer Training
- ACOT stands for Analog Channel Operational Test
- ACOT stands for American Civilization On Trial
ACOT