- AF1 stands for Air Force One
- AF-1 stands for Activation Function 1
- AF1 stands for Activation Function 1
- AF1 stands for Accessory Factor 1 (molecular biology)
- AF-1 stands for Axogenic Factor 1
- AF-1 stands for Fury Attack Aircraft (builty by Ryan)
- AF1 stands for Aircraft Maintenanceman First Class (Naval rating)
- AF-1 stands for AP (Activator Protein)-1-Associated Factor 1 (protein complex)
AF-1