- AFLS stands for Agricultural, Food and Life Sciences (Dale Bumpers College; Fayetteville, AR)
- AFLS stands for Association for French Language Studies
- AFLS stands for Accenture Freight and Logistics Software (various locations)
- AFLS stands for Adaptive Front Light System (automotive)
- AFLS stands for Armed Forces Language School
- AFLS stands for Administration, Financial, and Logistical Service
- AFLS stands for Academy of Foreign Language Study (China)
AFLS