- AFNI stands for Associazione Fotografi Naturalisti Italiani (Italian: Association of Italian Nature Photographers)
- AFNI stands for Anderson Financial Network, Inc.
- AFNI stands for Analysis of Functional Neuro-Images
- AFNI stands for American Forces Network, Iraq (American Forces Radio and Television Service)
- AFNI stands for Alaska Federation of Natives International
- AFNI stands for Adoptive Families of Northern Illinois (Rockford, IL)
- AFNI stands for Alachua FreeNet, Inc.
- AFNI stands for Associate Fellow of the Nautical Institute
AFNI