- AFO stands for Ankle-Foot Orthosis
- AFO stands for Animal Feeding Operation(s)
- AFO stands for Aquatic Facility Operator (various locations)
- AFO stands for Association Française des Orchestres (French: French Association of Orchestras)
- AFO stands for Association Française d’Ostéopathie (French: French Association of Osteopathy)
- AFO stands for Air Force One (The Movie)
- AFO stands for Arellano-Felix Organization (drug trafficking cartel)
- AFO stands for Algar-Flynn-Oyamada (chemical reaction)
AFO Alien Front Online (Sega video game)
AFO Admiralty Fleet Order
AFO Academy of Fixed Orthodontics (various locations)
AFO Air Force Ones (Nike shoes)
AFO Association Française d’Orthoptique (French: French Orthoptic Association; est. 1965)
AFO Authorised Firearms Officer (UK police)
AFO Advanced Furniture Outfitters (Bryan, OH)
AFO Armed Forces Only
AFO Advanced Force Operations (US DoD)
AFO Additional Forms
AFO Air Failure Open (valve operation mode)
AFO Alzheimer’s Family Organization (Florida)
AFO Axial Force Only (engineering)
AFO Auxiliary Fuel Oil
AFO Awaiting Further Occurrence
AFO Announced Flight Opportunity
AFO Announcement of Flight Opportunities
AFO Anti-Fascist Organization
AFO Advanced File Organizer (software)
AFO Accounting & Finance Office/r
AFO Alaska Field Office
AFO Audio Frequency Overlay (train location technology)
AFO Agency Field Office
AFO Aero Force One (Aerosmith fan club)
AFO Agencia Filatelica Oficial (Spanish: Official Philatelic Agency, Barcelona)
AFO Away from Office
AFO Available for Obligations
AFO Aircraft Flight Operations (job title)
AFO Alternate Fridays Off (work schedule)
AFO Army Finance Officer
AFO Area Finance Officer (various organizations)