- DKU stands for Donkey Kong Universe (game)
- DKU stands for Deutsch-Kasachische Universität (German: Kazakh-German University; Almaty, Kazakhstan)
- DKU stands for Danmarks Kommunistiske Ungdom (Danish: Denmark Communist Youth; 1906-1990)
- DKU stands for Dansk Kirke i Udlandet (Danish: Danish Church Abroad)
- DKU stands for Drop Kick Ugly
- DKU stands for Dewan Kuliah Utama (Malay: Main Lecture Hall)
DKU