- DLSI stands for De La Salle Institute (Malaysia)
- DLSI stands for Departament de Llenguatges i Sistemes Informàtics (Catalan: Department of Software and Computing Systems; University of Alicante; Spain)
- DLSI stands for Digital Library Service Integration
- DLSI stands for Davis Langdon and Seah International (various locations)
- DLSI stands for Drug Lifestyle Screening Interview (various locations)
DLSI