- DOTA stands for Defense of the Ancients (gaming)
- DOTA stands for Depending on the Artist (trope)
- DOTA stands for Doscientos Ocho Transporte Automotor (Buenos Aires bus line; Buenos Aires, Argentina)
- DOTA stands for Download over the Air (Wavecom Wireless)
- DOTA stands for Declaration of the Americas on Diabetes
- DOTA stands for Département d’Optique Théorique et Appliquée (French: Theoretical and Applied Optics Department)
- DOTA stands for Delaware Occupational Therapy Association
- DOTA stands for Department of Transportation, Airports Division (Hawaii)
DOTA