- DOTS stands for Department of Technology Services (various organizations)
- DOTS stands for Directly Observed Treatment Short-Course
- DOTS stands for Directly Observed Treatment Strategy
- DOTS stands for Difference of Two Squares (mathematics)
- DOTS stands for Dozens of Terrific Stamps
- DOTS stands for Database of Transcribed Sequences
- DOTS stands for Document Tracking System
- DOTS stands for Day of the Sword (band)
- DOTS stands for Dose-Responsiveness, Time-Course, Susceptibility Factors (used in classifying adverse drug reactions)
- DOTS stands for Dynamic Ocean Tracking System (US FAA)
- DOTS stands for Digital-Optical Technology System
- DOTS stands for Department of Trade Statistics
- DOTS stands for Directly Observed Treatment/Therapy Short Course
- DOTS stands for Deformities Open wounds Tenderness Swelling
- DOTS stands for Director Of Training and Simulations
- DOTS stands for Directory Order Transmission System
- DOTS stands for Dredging Operations Technical Services
DOTS