- DOTU stands for Disappearance of the Universe (TV series)
- DOTU stands for Dawn of the Undead (gaming clan)
- DOTU stands for Document the Undocumented (Cisco)
- DOTU stands for Defenders of the Universe (video game)
- DOTU stands for Down on the Upside (Soungarden album)
- DOTU stands for Drow of the Underdark (Dungeons and Dragons)
DOTU