- DTLS stands for Datagram Transport Layer Security (IETF)
- DTLS stands for Dans Tous Les Sens (French: In All Directions)
- DTLS stands for Descriptive Top-Level Specification
- DTLS stands for Division of Teaching and Learning Services (Australia)
- DTLS stands for Distributed Theater Level Simulation
- DTLS stands for Diagnostic Tests of Language Skills
- DTLS stands for Doctrine, Training, Leader, Soldiers
DTLS