- DUF stands for Dansk Ungdoms Fællesråd (Denmark)
- DUF stands for Domain of Unknown Function (protein)
- DUF stands for Drug Use Forecasting
- DUF stands for Discord Unit Frame
- DUF stands for Depleted Uranium Hexafluoride
- DUF stands for Daily Usage File
- DUF stands for Design Up Front (software design methodology)
- DUF stands for Diffusion under Film
- DUF stands for Durham Ultimate Frisbee (UK)
- DUF stands for Dictionnaire Universel Francophone (French: Universal French Dictionary)
- DUF stands for Designated Ugly Friend
DUF